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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, and immunoprecipitation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 105708).
Application References:
1. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP) 2. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) 3. Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC) 4. Kinashi T, et al. 1994. Blood Cells 20:25. (Block) 5. Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC) 6. Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC) 7. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) 8. Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed
C57BL/6 bone marrow myeloid cells stained with 429 Alexa Fluor® 647
CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD106
C57BL/6 bone marrow myeloid cells
stained with 429 FITC
FITC anti-mouse CD106
C57BL/6 bone marrow myeloid cells
stained with 429 FITC
LEAF™ Purified anti-mouse CD106
C57BL/6 bone marrow myeloid cells
stained with 429 FITC
Purified anti-mouse CD106
C57BL/6 mouse bone marrow myeloid cells stained with 429 purified, followed by anti-rat IgG FITC.
Alexa Fluor® 488 anti-mouse CD106
C57BL/6 mouse bone marrow cells stained with 429 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD106
C57BL/6 bone marrow myeloid cells stained with 429 Alexa Fluor® 647
PE anti-mouse CD106
C57BL/6 bone marrow cells stained with 429 PE (gated on myeloid cell population)
PerCP/Cy5.5 anti-mouse CD106
C57BL/6 bone marrow cells stained with 429 PerCP/Cy5.5 (gated on myeloid cell population)
APC anti-mouse CD106
C57BL/6 mouse bone marrow cells stained with 429 APC
PE/Cy7 anti-mouse CD106
C57BL/6 bone marrow cells stained with 429 PE/Cy7
Pacific Blue™ anti-mouse CD106
C57BL/6 bone marrow cells stained with 429 Pacific Blue™ and CD11b (M1/70) PE
C57BL/6 bone marrow cells stained with rat IgG2a isotype control Pacific Blue™ and CD11b (M1/70) PE
