The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
ICFC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application References:
1. Krensky, A.M. et al. 1997, Methods Enzymol 287:162
Human PBMCs cultured overnight with monensin, then surface stained with CD3 PE and intracellularly stained with VL1 Alexa Fluor® 647 (top panel), lower panel data shows the staining was specifically blocked by pre-incubation with purified VL1 antibody (gated on lymphocyte population)
Description:
RANTES (CCL5) is a member of the CC chemokine family. Its mature form consists of 68 amino acid residues. RANTES is a strong chemotractant for monocytes, NK cells, T cells and eosinphils. Rantes may have important role in mediating immune and inflammatory responses.
Other Names:
CCL5, C-C motif chemokine 5, Small-inducible cytokine A5, T-cell-specific protein RANTES, SIS-delta, T cell-specific protein P228, TCP228, Eosinophil-chemotactic cytokine, EoCP
Structure:
Human RANTES cDNA is a member of the C-C cehmokine family with a MW of around 8kd. Human and mouse RANTES have 85% homology.
Distribution:
Reported to be produced by T cells, platelets, synovial fibroblasts and some tumor cells.
Function:
Able to chemoattract monocytes, NK cells, esosinphils and T cells.
Interaction:
Monocytes, NK, T and eosinphils.
Antigen References:
1. Von, Luettichau. et al. 1996. Cytokine 8:89 2. Schall, T.J. et al. 1991. Cytokine 3:165
