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Alexa Fluor® 647 anti-human IL-21 Antibody
Alexa Fluor® 647 anti-human IL-21 Antibody
513005 25 tests $150.00     
513006 100 tests $305.00     
Product Details
Clone: 3A3-N2
Isotype: Mouse IgG1, κ
Isotype Control:Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human
Immunogen: Recombinant full length human IL-21
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.



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 PMA/ionomycin-stimulated (6 hours) human peripheral
PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellular stained with 3A3-N2 Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom) and CD3 (UCHT1) PE




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Description:

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses.These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells.  It can also be proapoptotic for B cells and NK cells.Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces T(H)17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.
Other Names: Interleukin 21
Distribution: Th17
Function: Immunomodulation
Cellular Sources: NK-T cells and T cells
Cellular Targets: cytotoxic T cells (CTL) and Natural Killer (NK) cells, that can destroy malignant or infected cells
Receptors: IL-21R
Antigen
References:

1. Nurieva R. 2007. Nature 448:416.
2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856.
3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao H, et al. 2001. J. Immunol. 167:1.
5. Parrish-Novak J, et al. 2000. Nature 408:57.
GeneID: 59067
Latest Publications: View the latest IL-21 articles on HighwirePress.com
UniProt: View information about IL-21 on UniProt.org
Keywords: Alexa Fluor® 647 anti-human IL-21, 3A3-N2, Alexa Fluor® 647, Interleukin 21, Human, Immunology, Antibodies
Related Products
DescriptionCloneApplications
Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC)MOPC-21FC
Cell Staining BufferFC, ICC, ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-human IL-21




  • PE anti-human IL-21
    PMA+ionomycin-stimulated (5 hours) human PBMCs

    PMA+ionomycin-stimulated (5 hours) human PBMCs intracellular stained with 3A3-N2 PE and CD3 APC (top panel), bottom data shows the staining of 3A3-N2 PE can be blocked by preincubation of the cells with purified 3A3-N2





  • Alexa Fluor® 647 anti-human IL-21
    PMA/ionomycin-stimulated (6 hours) human peripheral

    PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellular stained with 3A3-N2 Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom) and CD3 (UCHT1) PE





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