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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
ELISA and ELISPOT Capture: The purified JK1B-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JK1B-2 antibody (Cat. No. 508301) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled JK1B-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1β-producing cells within mixed cell populations. View intracellular cytokine staining protocol
LPS-stimulated (6 hour) human peripheral blood monocytes surface stained with CD14 PE, then intracellualar stained with JK1B-1 Alexa Fluor® 647
IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The JK1B-1 antibody reacts with human interleukin-1β (IL-1β) and baboon IL-1β. The JK1B-1 antibody does not recognize mouse IL-1β or human IL-1α or other cytokines. The JK1B-1 antibody can neutralize the bioactivity of natural or recombinant IL-1β.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human IL-1β
FITC anti-human IL-1β
LP-stimulated (6 hours) human peripheral blood monocytes surface stained with CD14 APC then intracellular stained with JK1B-1 FITC (top) or mIgG1,k isotype control FITC (bottom)
Alexa Fluor® 647 anti-human IL-1β
LPS-stimulated (6 hour) human peripheral blood monocytes surface stained with CD14 PE, then intracellualar stained with JK1B-1 Alexa Fluor® 647
