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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
ELISA Capture1-5 or ELISPOT Capture6: The purified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 561201) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization1-3,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. No. 501407). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430601 to 430606) are specially developed and recommended.
Application References:
1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut) 2. Gotlieb W, et al. 1992. Cytokine 4:385. (ELISA Capture, Neut) 3. Yssel H, et al. 1992. J. Immunol. 149:2378. (ELISA Capture, Neut) 4. Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20. (ELISA Capture) 5. Burdin N, et al. 1993. J. Exp. Med. 177:295. (ELISA Capture) 6. Klinman D, et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.19. (ELISPOT Capture) 7. Schaerli P, et al. 2000. J. Exp. Med. 192:1553. 8. Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73. 9. Akdis CA, et al. 1998. J. Clin. Invest. 102:98. (Neut) 10. Stary G, et al. 2011. J. Immunol. 186:103. PubMed
LPS-stimulated (overnight) human peripheral blood monocytes surface stained with with CD14 (M5E2) PE and intracellularly stained with JES3-9D7 Alexa Fluor® 647
IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).
Other Names:
Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)
Structure:
Acid-labile cytokine; dimer; 35-40 kD (Mammalian)
Regulation:
Production inhibited by IL-4, IL-10
Cellular Sources:
Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
Cellular Targets:
T cells, B cells, mast cells, macrophages
Receptors:
IL-10R (CDw210)
Bioactivity/Activities:
Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314. 3. Howard M, et al. 1992. Immunol. Today. 13:198. 4. Quesniaux V. 1992. Research Immunol. 143:385.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
LEAF™ Purified anti-human IL-10
PE anti-human IL-10
LPS-stimulated (20 hours) human monocytes were surface stained with CD14 FITC and then intracellular stained with JES3-9D7 PE
Purified anti-human IL-10
APC anti-human IL-10
LPS-stimulated (overnight) human peripheral blood monocytes surface stained with with CD14 (M5E2) PE and intracellularly stained with JES3-9D7 APC
Alexa Fluor® 488 anti-human IL-10
LPS-stimulated (overnight) human peripheral blood monocytes surface stained with with CD14 (M5E2) PE and intracellularly stained with JES3-9D7 Alexa Fluor® 488
Alexa Fluor® 647 anti-human IL-10
LPS-stimulated (overnight) human peripheral blood monocytes surface stained with with CD14 (M5E2) PE and intracellularly stained with JES3-9D7 Alexa Fluor® 647
PE/Cy7 anti-human IL-10
LPS-stimulated (overnight) human peripheral blood mononuclear cells surface stained with CD14 APC and intracellularly stained with JES3-9D7 PE/Cy7 (top data) or rat IgG1 isotype control PE/Cy7 (bottom data) (gated on monocyte population)
Brilliant Violet 421™ anti-human IL-10
Human peripheral blood mononuclear cells were stimulated overnight with LPS (in the presence of monensin), stained with CD14 FITC, fixed, permeabilized, and then stained with IL-10 (clone JES3-9D7) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on monocyte population.
