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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions7,9,10, and immunohistochemical staining of acetone-fixed frozen sections4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 307612).
Application References:
1. Brodsky F. 1984. Immunogenetics 19:179. 2. Robbins P, et al. 1987. Human Immunol. 18:301. 3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161. 4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC) 5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC) 6. Zipf T, et al. 1981. Cancer Res. 41:4786. 7. Goodier M, et al. 2000. J. Immunol. 165:139. (Block) 8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB) 9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:1649. (Block) 10. Wang RF, et al. 1999. Science 284:1351. (Block) 11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed 12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed 13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 14. Goncalves RM, et al. 2010. Infect. Immun. 128:578. PubMed 15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
Human peripheral blood lymphocytes stained with L243 Alexa Fluor® 647
HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
Other Names:
Major Histocompatibility Class II, MHC class II
Structure:
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD
Distribution:
B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
Function:
Peptide presentation
Ligand Receptor:
CD3/TCR, CD4
Antigen References:
1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177. 2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138. 3. Edwards J, et al. 1985. J. Immunol. 137:490. 4. van Es A, et al. 1984. Transplantation 37:65. 5. O'Doherty U, et al. 1994. Immunology 82:487. 6. Thomas R, et al. 1994. J. Immunol. 153:4016. 7. Grouard G, et al. 1996. Nature 384:364.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 APC
FITC anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 FITC
LEAF™ Purified anti-human HLA-DR
Human peripheral blood lymphocytes stained with LEAF™ purified L243, followed by anti-mouse IgG FITC
PE anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE
PE/Cy5 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE/Cy5
Purified anti-human HLA-DR
Human peripheral blood lymphocytes stained
with L243 PE
Biotin anti-human HLA-DR
Human peripheral blood lymphocytes stained with biotinylated L243, followed by Sav-PE
PE/Cy7 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE/Cy7
APC/Cy7 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 APC/Cy7
Alexa Fluor® 488 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa Fluor® 488
Alexa Fluor® 647 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa Fluor® 647
Pacific Blue™ anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Pacific Blue™
Alexa Fluor® 700 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa Fluor® 700
PerCP anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PerCP
PerCP/Cy5.5 anti-human HLA-DR
Human peripheral blood lymphocytes with L243 PerCP/Cy5.5
Brilliant Violet 421™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR (clone L243) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR (clone L243) Brilliant Violet 570™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 570™ isotype control (open histogram).
