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Alexa Fluor® 647 anti-human/mouse Granzyme B Antibody
Alexa Fluor® 647 anti-human/mouse Granzyme B Antibody
The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Wever PC, et al. 1998. Immunology. 93:383 2. Arens R, et al. 2004. J. Exp. Med. 199:1595 3. Lima M, et al. 2003. Am. J. Pathol. 163:763
Human peripheral blood lymphocytes were surface stained with CD8 PE, then intracellularly stained with Granzyme B (clone GB11) Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (middle).
C57BL/6 mouse splenocytes were stimulated with plate-bound anti-mouse TCR-β for 2 days, then surface stained with CD8 PE and intracellularly stained with Granzyme B (clone GB11) Alexa Fluor® 647.
Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells
Function:
Induction of cell death and apoptosis
Ligand Receptor:
mannose-6-phosphate receptor
Antigen References:
1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203 2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885 3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610 4. Wagner C, et al. 2008. Mol. Immunol. 45:1761
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human/mouse Granzyme B
Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with GB11 FITC
Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with mouse IgG1 FITC isotype control
Alexa Fluor® 647 anti-human/mouse Granzyme B
Human peripheral blood lymphocytes were surface stained with CD8 PE, then intracellularly stained with Granzyme B (clone GB11) Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (middle).
C57BL/6 mouse splenocytes were stimulated with plate-bound anti-mouse TCR-β for 2 days, then surface stained with CD8 PE and intracellularly stained with Granzyme B (clone GB11) Alexa Fluor® 647.
