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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Hanson DA, et al. 1999. Mol. Immunol. 36:413. 2. Spada FM, et al. 2000. J. Exp. Med. 191:937.
Human peripheral blood lymphocytes surface stained with CD56 PE then intracellularly stained with DH2 Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom)
Granulysin is a member of the saposin-like protein family of lipid binding proteins (SAPLIP). It is expressed by NK cells and cytolytic T lymphocytes (CTL) as 9 kD and 15 kD forms. The 9 kD form is derived from the 15 kD form by cleavage at both amino- and carboxy-termini. Granulysin has broad cytolytic activities against a variety of tumor cells and microbes, including Gram-positive and -negative bacteria, fungi and parasites. It also functions as a chemoattractant for inflammatory cells and activates the cells expression of cytokines and chemokines.
Structure:
Saposin-like protein family of lipid binding protein, 9 kD and 15 kD
Distribution:
NK cells, cytolytic T cells
Function:
Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Antigen References:
1. Krensky AM. 2000. Biochem. Pharmacol. 59:317. 2. Deng A, et al. 2005. J. Immunol. 174:5243. 3. Kaspar AA, et al. 2001. J. Immunol. 167:350. 4. Krensky AM and Clayberger C. 2009. Tissue Antigens 73:193.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human Granulysin
Human peripheral blood lymphocytes stained with CD8 (RPA-T8) APC then intracellular stained with DH2 PE (top) or mIgG1,k PE isotype control (bottom)
Purified anti-human Granulysin
Human peripheral blood lymphocytes stained with CD8 (RPA-T8) APC then intracellular stained with DH2 PE (top) or mIgG1,k PE isotype control (bottom)
Alexa Fluor® 647 anti-human Granulysin
Human peripheral blood lymphocytes surface stained with CD56 PE then intracellularly stained with DH2 Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom)
