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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Yamaguchi M, et al. 1999. J. Immunol. 162:5455 2. Suzukawa M, et al. 2005. Int. Immunol. 17:1249. 3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Human peripheral blood leukocytes stained with CD203c (NP4D6) PE and AER-37 (CRA1) Alexa Fluor® 647 (gated on lymphocyte population)
High affinity IgE receptor (FcεRI) plays a key role in IgE-mediated allergic immune response. FcεRI is a tetrameric receptor complex, which is composed of one α-subunit (FcεRIα), one β-subunit, and two γ-subunits. FcεRIα directlybinds IgE with high affinity, while the β- and γ-chains are responsible for mediatingintracellular signals. FcεRIα is a 50 kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FcεRIα is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.
Other Names:
FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor
Structure:
Ig superfamily, 50 kD
Distribution:
Mast cells, basophils, cutaneuos Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients
Function:
Bind IgE, trigger IgE-mediated allergic response
Ligand Receptor:
IgE
Antigen References:
1. Riske F, et al. 1991. J. Biol. Chem. 266:11245 2. Gounni AS, et al. 2001. FASEB J. 15:940. 3. Maurer D, et al. 1996. J. Immunol. 157:607 4. Maurer d, et al. 1994. J. Exp. Med. 179:745 5. Campbell AM, et al. 1998. Am. J. Respir. Cell Mol. Biol. 19:92.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human FcεRIα
Human peripheral blood lymphocytes stained with purified AER-37, followed by anti-mouse IgG FITC
FITC anti-human FcεRIα
Human peripheral blood leukocytes stained with CD203c (NP4D6) PE and AER-37 (CRA1) FITC (gated on lymphocyte population)
PE anti-human FcεRIα
Human peripheral blood leukocytes stained with AER-37 (CRA-1) PE (gated on lymphocyte population)
Alexa Fluor® 647 anti-human FcεRIα
Human peripheral blood leukocytes stained with CD203c (NP4D6) PE and AER-37 (CRA1) Alexa Fluor® 647 (gated on lymphocyte population)
PerCP anti-human FcεRIα
Human peripheral blood leukocytes stained with CD203c (NP4D6) PE and AER-37 (CRA1) PerCP (gated on lymphocyte population)
APC anti-human FcεRIα
Human peripheral blood lymphocytes stained with CD203c (NP4D6) PE and AER-37 APC (top) or mouse IgG2b, κ APC isotype control(bottom)
Pacific Blue™ anti-human FcεRIα
Human peripheral leukocytes stained with CD203c (NP4D6) PE and AER-37 Pacific Blue™ (top) or mouse IgG2b, κ Pacific Blue™ isotype control(bottom) (gated on lymphocyte population)
PE/Cy7 anti-human FcεRIα
Human peripheral blood leukocytes stained with CD203c (NP4D6) PE and AER-37 PE/Cy7 (top) or mouse IgG2b, κ PE/Cy7 isotype control (bottom) (gated on lymphocyte population)
PerCP/Cy5.5 anti-human FcεRIα
Human peripheral blood lymphocytes were stained with CD203c PE and FcεRIα (clone AER-37) PerCP/Cy5.5 (top) or mouse IgG2b, κ PerCP/Cy5.5 isotype control(bottom).
