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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4.
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York. 2. Zhou L, et al. 1995. J. Immunol. 154:3821. 3. Cao W, et al. 2005. Biochem. J. 385:85. 4. Lore K, et al. 2002. AIDS 16:683. (IHC) 5. Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006
Human monocytes-derived dendritic cells stained with HB15e Alexa Fluor® 647
CD83 is a 43 kD single chain type I glycoprotein also known as HB15. A member of the immunoglobulin superfamily, CD83 is expressed on a subset of dendritic cells, Langerhans cells, and weakly on activated lymphocytes. Although CD83 is thought to play a role in antigen presentation and/or lymphocyte activation, the precise function of this protein is unknown. CD83 is considered to be a useful marker for mature dendritic cells.
Other Names:
HB15
Structure:
Ig superfamily, single chain transmembrane glycoprotein, 43 kD
Distribution:
Dendritic cells, Langerhan cells, activated B and T cells
Antigen References:
1. Kozlow E, et al. 1993. Blood 81:454. 2. Zhou L, et al. 1992. J. Immunol. 149:735. 3. Zhou L, et al. 1995. Blood 86:3295.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD83
Monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-?) stained with HB15e APC
Biotin anti-human CD83
Monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-?) stained with biotinylated HB15e, followed by Sav-PE
FITC anti-human CD83
Human monocyte-derived dendritic cells stained with HB15e FITC
PE anti-human CD83
Monocyte-derived dendritic cells
stained with HB15e PE
PE/Cy5 anti-human CD83
Monocyte-derived dendritic cells (induced with GM-CSF+IL-4+TNF-?) stained with HB15e PE/Cy5
Purified anti-human CD83
Monocyte-derived dendritic cells
stained with HB15e PE
Alexa Fluor® 488 anti-human CD83
Human monocytes-derived mature dendritic cells stained with HB15e Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD83
Human monocytes-derived dendritic cells stained with HB15e Alexa Fluor® 647
PerCP/Cy5.5 anti-human CD83
Human monocyte-derived dendritic cells stained with HB15e PerCP/Cy5.5
Brilliant Violet 421™ anti-human CD83
Human monocyte-derived dendritic cells (induced with GM-CSF+IL-4+TNF-a) were stained with CD83 (clone HB15e) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
