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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro blocking of adhesion of platelets1. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 304912).
Application References:
1. Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block) 2. Kishimoto T , et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. 3. Yen Y-T, et al. 2006. J. Virolo. 80:2684.
Thrombin-activated human platelets stained with AK4 Alexa Fluor® 647
CD62P is a 140 kD type I transmembrane glycoprotein also known as P-Selectin, platelet activation-dependent granule membrane protein (PADGEM), or GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62 is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, platelet-neutrophil, and platelet-monocyte interactions.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained with AK4 APC
FITC anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained with AK4 FITC
LEAF™ Purified anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained with LEAF™ purified AK4, followed by anti-mouse IgGs FITC
PE anti-human CD62P (P-Selectin)
Thrombin-activated human platelets stained with AK4 PE
PE/Cy5 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained with AK4 PE/Cy5
Purified anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained with purified AK4, followed by anti-mouse IgGs FITC
Biotin anti-human CD62P (P-Selectin)
Thrombin-activated human platelets stained with biotinylated AK4, followed by Sav-PE
Alexa Fluor® 488 anti-human CD62P (P-Selectin)
Thrombin-activated human peripheral blood platelets stained with AK4 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD62P (P-Selectin)
Thrombin-activated human platelets stained with AK4 Alexa Fluor® 647
