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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L, and B cell rescue from apoptosis1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.
CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.
Other Names:
BP50, TNFRSF5
Structure:
TNFR superfamily, type I glycoprotein, 48 kD
Distribution:
B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
Function:
B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Ligand Receptor:
CD154 (CD40 ligand)
Antigen References:
1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881. 2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 488 anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 Alexa Fluor® 488
Purified anti-human CD40
Human peripheral blood lymphocytes stained with purified 5C3, followed by anti-mouse IgG FITC
LEAF™ Purified anti-human CD40
Human peripheral blood lymphocytes stained with LEAF™ purified 5C3, followed by anti-mouse IgG FITC
FITC anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 FITC
PE anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 PE
APC anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 APC
Alexa Fluor® 647 anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 Alexa Fluor® 647
PerCP/Cy5.5 anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 PerCP/Cy5.5
Pacific Blue™ anti-human CD40
Human peripheral blood lymphocytes stained with 5C3 Pacific Blue™
PE/Cy7 anti-human CD40
Human peripheral blood lymphocytes stained with CD19 (HIB19) FITC and 5C3 PE/Cy7 (top) or mouse IgG1, κ PE/Cy7 isotype control (bottom)
APC/Cy7 anti-human CD40
Human peripheral blood lymphocytes stained with CD19 (HIB19) FITC and 5C3 APC/Cy7
