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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.
Application References:
1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. 2. Dieu M. 1998. J. Exp. Med. 188:373. 3. Esser M, et al. 2001. J. Virol. 75:6173. 4. Jeannin P, et al. 1999. J. Immunol. 162:2044. 5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. 6. van der Voort R, et al. 1997. J. Exp. Med. 185:2121. (IHC) 7. Bende RJ, et al. 2003. Am. J. Pathol. 162:105. 8. Lehner M, et al. 2008. J. Leukoc. Biol. 83:883. PubMed 9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained with HIT2 Alexa Fluor® 647
CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation as well as adhesion and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
Other Names:
T10, ADP-ribosyl cyclase
Structure:
ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 APC
FITC anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 FITC
PE anti-human CD38
Human peripheral blood lymphocytes stained
with HIT2 PE
PE/Cy5 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PE/Cy5
Purified anti-human CD38
Human peripheral blood lymphocytes stained with purified HIT2, followed by anti-mouse IgGs FITC
Alexa Fluor® 488 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 Alexa Fluor® 647
PE/Cy7 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PE/Cy7
Biotin anti-human CD38
Human peripheral blood lymphocytes stained with biotinylated HIT2, followed by Sav-PE
PerCP anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PerCP
PerCP/Cy5.5 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PerCP/Cy5.5
Alexa Fluor® 700 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 Alexa Fluor® 700
Brilliant Violet 421™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (clone HIT2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
