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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The 24D2 recognizes human CD340 also known as HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, and oncogene NGL, neuroblastoma- or glioblastoma-derived. CD340 is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain with an approximate molecular weight of 185 kD. CD340 Contains three furin repeats and two cheY homologous receiver domains in the extracellular region. The HER2/erbB2 protein is expressed on many tumor cells including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, gliblastoma, some c-ALL blasts and bone marrow MSC. Although the erbB2/HER2 protein contains no ligand binding domain, this protein interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Over-expression of erbB2 in breast cancers has been shown to confer Taxol resistance and is a therapeutic target in a number of human cancers. The erbB2/HER-2 protein has been shown to interact with a large number of proteins including SHC1, EGF receptorm, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others. CD340 is extensively modified by tyrosine phosphoryation on multiple residues (Y 1023, Y1139, Y1196, Y1221, Y1222, Y1248). The 24D2 antibody has been shown to be useful for flow cytometry.
Member of the epidermal growth factor receptor family of cell membrane tyrosine kinases. Single transmembrane pass protein with an approximate molecular weight of 185 kD. Contains three furin repeats and two cheY homologous receiver domains in the extrace
Distribution:
Expressed on many tumor cells including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, gliblastoma, some c-ALL blasts and bone marrow MSC
Function:
Contains no ligand binding domain and interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. Involved in embryonic development and cancer progression.
Modification:
Extensively modified by tyrosine phosphoryation on multiple residues (Y 1023, Y1139, Y1196, Y1221, Y1222, Y1248)
Interaction:
CD340 (HER2/erbB2) interacts with a large number of proteins including SHC1, EGF receptorm, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others
Antigen References:
1. Akiyama T, et al. 1986. Science 232:1644. 2. Bargmann CI, et al. 1986. Nature 319:226. 3. Pegram MD, et al. 1997. Oncogene 15:537. 4. Slamon DJ, et al. 1989. Science 244:707. 5. Leone F, et al. 2003. J. Leukocyte Biol. 74:593.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD340 (erbB2/HER-2)
Human breast cancer cell line (MCF-7) stained with purified 24D2, followed by anti-mouse IgG FITC
FITC anti-human CD340 (erbB2/HER-2)
Human breast cancer cell line (MCF-7) stained with 24D2 FITC
PE anti-human CD340 (erbB2/HER-2)
Human breast adenocarcinoma cell line MCF-7 stained with 24D2 PE
APC anti-human CD340 (erbB2/HER-2)
Human breast cancer cell line (MCF-7) stained with 24D2 APC
Alexa Fluor® 488 anti-human CD340 (erbB2/HER-2)
Human breast adenocarcinoma cell line MCF-7 stained with 24D2 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD340 (erbB2/HER-2)
Human breast cancer cell line (MCF-7) stained with 24D2 Alexa Fluor® 647
