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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
CD338, known as ABCG2 (BCRP1), is a multi-drug resistance (MDR) family protein that is a member of ATP binding cassette (ABC) transporters. It is highly expressed on primitive ‘side population’ (SP) stem cells. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. The SP cells express low or undetectable levels of CD34. In the bone marrow, about 0.05% population of cells displays the low fluorescence and are highly enriched for repopulating cells. The highest levels of BCRP1 mRNA expression have been seen in KDR+ human stem cells. The expression of ABCG2 appears to be highly conserved as it has been identified in various species.
Other Names:
ABCG2, CDw338, Bcrp1, MXR
Distribution:
primitive ‘sidepopulation’ (SP) stem cells, KDR+ human stem cells
Antigen References:
1. Kim M, et al. 2002. Clin. Cancer Res. 8:228. 2. Scharenber CW, et al. 2002. Blood 99:50712. 3.Goodell MA, et al. 1997. Nat. Med. 3:133745. 4. Bunting KD. 2002. Stem Cells. 20:11.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 647 anti-human CD338 (ABCG2)
ABCG2 transfected cells stained with 5D3 Alexa Fluor® 647
Purified anti-human CD338 (ABCG2)
Human ABCG2 transfected cells stained with purified 5D3, followed by anti-mouse IgG FITC
Biotin anti-human CD338 (ABCG2)
Human ABCG2 transfected cells stained with 5D3 biotin, followed by Sav-PE
PE anti-human CD338 (ABCG2)
Human ABCG2 transfected cells stained with 5D3 PE
Pacific Blue™ anti-human CD338 (ABCG2)
ABCG2 transfected cells stained with 5D3 Pacific Blue™
FITC anti-human CD338 (ABCG2)
ABCG2 transfected cells stained with 5D3 FITC
PerCP/Cy5.5 anti-human CD338 (ABCG2)
Human ABCG2 transfected cells stained with 5D3 PerCP/Cy5.5
APC anti-human CD338 (ABCG2)
Human ABCG2 transfected cells stained with 5D3 APC
