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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The p44-8 antibody against human NKp44 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution. Additional reported applications (for the relevant formats) include: ELISA, stimulation of human NK cells via NKp44 in a redirected lysis assay, and blocking of NKp44 function in solution1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 325104).
Application References:
1. Stark S, et al. 2005. J. Immunol. Methods 296:149. 2. Nolte-'t Hoen E, et al. 2006. Blood DOI10.1182/blood-2006-07-036509
Human PBMCs were stimulated with rhIL-2 for 9 days, then stained with CD56 PE and P44-8 Alexa Fluor® 647
CD336 is also known as activating NK receptor NKp44 (NKp44), natural cytotoxicity triggering receptor 2, and lymphocyte antigen 95 homolog. It is a type I transmembrane protein, member of the natural cytotoxicity receptor family that contains one immunoglobulin-like domain. NKp44 has an apparent molecular weight of 44 kD and three isoforms are produced by alternative splicing. NKp44 is expressed on IL-2 activated NK cells and a subset of γ/δ T cells. NKp44 enhances NK cell mediated cytolysis of virus infected cells and tumor cells. NKp44 has been shown to associate with the intracellular adaptor DAP12.
Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Three isoforms produced by alternative splicing.
Distribution:
IL-2 activated NK cells and cell lines, some γ/δ T cells activated in vitro
Function:
NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction:
DAP12
Antigen References:
1. Cantoni C, et al. 1999. J. Exp. Med. 189:787. 2. Allcock RJN, et al. 2003. Eur. J. Immunol. 33:567. 3. Cantoni C, et al. 2003. Structure 11:725.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD336 (NKp44)
Human PBMCs were stimulated with rhIL-2 for 9 days, then stained with CD56 APC and biotinylated P44-8, followed by Sav-PE
PE anti-human CD336 (NKp44)
Human PBMCs were stimulated with rhIL-2 for 7 days, then stained with P44-8 PE and CD56 PE/Cy5
APC anti-human CD336 (NKp44)
Human PBMCs were stimulated with rhIL-2 for 7 days, then stained with P44-8 APC and CD56 PE
Alexa Fluor® 647 anti-human CD336 (NKp44)
Human PBMCs were stimulated with rhIL-2 for 9 days, then stained with CD56 PE and P44-8 Alexa Fluor® 647
