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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Nagata K, et al. 1999. J. Immunol. 162:1278. (FC) 2. De Fanis U, et al. 2007. Blood 109:4343. (FC) 3. Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC) 4. Hamada K, et al. 2004. Allergology International 53:179. (FC)
Human peripheral blood lymphocytes stained with CCR4 PerCP/Cy5.5 and BM16 Alexa Fluor® 647 (top) or rat IgG2a Alexa Fluor® 647 isotype control (bottom) (gated on CD4+ lymphocyte population)
Human Th2 polarized CD4+ T cells stained with BM16 Alexa Fluor® 647
CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.
Seven-transmembrane protein coupled with G proteins
Distribution:
Effector T helper 2 (Th2) cells, eosinophils, basophils
Function:
Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
Ligand Receptor:
Prostaglandin D2
Interaction:
Heterotrimeric G proteins
Antigen References:
1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711. 2. Monneret G, et al. 2001. Blood 98:1942. 3. Xue L, et al. 2009. J. Immunol. 182:7580. 4. Schratl P, et al. 2007. J. Immunol. 179:4792.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes stained with CCR4 PerCP/Cy5.5 and purified BM16 conjugated with Alexa Fluor® 647 (top) or rat IgG2a Alexa Fluor® 647 isotype control (bottom) (gated on CD4+ lymphocyte population)
Human Th2 polarized CD4+ T cells stained with purified BM16 conjugated with Alexa Fluor® 647
Alexa Fluor® 647 anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes stained with CCR4 PerCP/Cy5.5 and BM16 Alexa Fluor® 647 (top) or rat IgG2a Alexa Fluor® 647 isotype control (bottom) (gated on CD4+ lymphocyte population)
Human Th2 polarized CD4+ T cells stained with BM16 Alexa Fluor® 647
PE anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 APC, CCR4 PerCP/Cy5.5, and CD294 (clone BM16) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown was gated on the CD4+ cell population.
FITC anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 PE, CCR4 PerCP/Cy5.5, and CD294 (clone BM16) FITC (top) or rat IgG2a, κ FITC isotype control (bottom). Data shown was gated on the CD4+ population.
APC anti-human CD294 (CRTH2)
Human peripheral blood lymphocytes were stained with CD4 PE, CCR4 PerCP/Cy5.5, and CD294 (clone BM16) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown was gated on the CD4+ population.
