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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation2,3, and blocking of cytokine production and inhibition of T- and NK-cell-mediated cytotoxicity1,2.
Application References:
1. Shibuya A, et al. 1996. Immunity 4(6):573. 2. Shibuya A, et al. 1998. J. Immunol. 161:1671. 3. Kojima H, et al. 2003. J. Biol. Chem. 278:36748.
Human peripheral blood lymphocytes stained with DX11 Alexa Fluor® 647
CD226 is a 65 kD type I transmembrane glycoprotein, known as DNAM-1 (DNAX accessory molecule-1), PTA1(platelet and T cell activation antigen 1), or TLiSA1 (T lineage-specific activation antigen 1 antigen). It belongs to the immunoglobulin superfamily containing 2 Ig-like domain and is expressed on majority of T lymphocytes, NK cells, monocytes, and platelets as well as a subset of B cells and activated endothelial cells. DNAM-1 is also expressed on CD8+ (but not CD8-) dendritic cells in mouse model. CD226 is an adhesion molecule involved in CTL and NK cell-mediated cytotoxicity. It has been identified that CD155 (poliovirus receptor, PVR) and CD112 (nectin-2, PRR-2) are CD226 ligands. DX11 antibody is able to inhibit CTL and NK cell-mediated cytotoxicity, and to block T cell cytokine production.
Other Names:
DNAM-1 (DNAX Accessory Molecule-1); PTA1; TLiSA1
Structure:
Ig superfamily, type I transmembrane glycoprotein, 65kD
Distribution:
Majority of T cells, NK cells, monocytes, platelets, subset of B cells
Function:
Mediate cell adhesion, involving in CTL and NK cell-mediated cytotoxicity
Ligand Receptor:
CD155 (PVR), CD112 (Nectin-2)
Antigen References:
1. Shibuya A, et al. 1996. Immunity 4(6):573. 2. Chen L, et al. 2003. Life Sci. 73(18):2373. 3. Bottino C, et al. 2003. J. Exp. Med. 198(4):557. 4. Tahara-Hanaoka S, et al. 2006. Blood 107(4):1491.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD226 (DNAM-1)
Human peripheral blood lymphocytes stained with purified DX11, followed by anti-mouse IgGs FITC
Alexa Fluor® 647 anti-human CD226 (DNAM-1)
Human peripheral blood lymphocytes stained with DX11 Alexa Fluor® 647
