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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
CD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.
Other Names:
Tie2, TEK
Structure:
145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution:
Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes
Function:
Angiogenesis, hemotopoiesis, monocytes chemotaxis
Ligand Receptor:
Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Antigen References:
1.De Palma M, et al. 2005. Cancer Cell. 8:211 2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168 3. Hsu HC, et al. 2000. Blood. 96:3757 4. Arai F, et al. 2004. Cell. 118:149
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD202b (Tie2/Tek)
Human endothelial cells (HUVEC) stained with purified AB33, followed by biotinylated anti-mouse IgG and Sav-PE
Biotin anti-human CD202b (Tie2/Tek)
Human endothelial cell line, HUVEC, stained with biotinylated 33.1, followed by Sav-PE
PE anti-human CD202b (Tie2/Tek)
Human endothelial cell line, HUVEC, stained with 33.1 PE
Alexa Fluor® 488 anti-human CD202b (Tie2/Tek)
Human endothelial cell line, HUVEC, stained with 33.1 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD202b (Tie2/Tek)
Human endothelial cell line, HUVEC, stained with 33.1 Alexa Fluor® 647
