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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation4, immunohistochemical staining5 of acetone-fixed frozen sections.
Application References:
1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York. 4. Polyak MJ, et al. 2002. Blood 99:3256. (IP) 5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 647 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 647 (open histogram).
CD20 is a 33-37 kD four transmembrane spanning protein also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn), and exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Other Names:
B1, Bp35
Structure:
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution:
B cell, T cell subsets
Function:
B cell activation
Ligand Receptor:
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Antigen References:
1. Hultin L, et al. 1993. Cytometry 14:196. 2. Tedder T, et al. 1994. Immunol. Today 15:450.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC (filled histogram), or mouse IgG2b, κ APC (open histogram).
FITC anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) FITC (dark line), or mouse IgG2b, κ FITC (dashed line).
PE anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE (solid line), or mouse IgG2b, κ PE (dashed line).
PE/Cy5 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE/Cy5 (solid line), or mouse IgG2b, κ PE/Cy5 (dashed line).
Purified anti-human CD20
Human peripheral blood lymphocytes were stained with purified anti-CD20 (clone 2H7) (solid line), or mouse IgG2b, κ (dashed line) followed by anti-mouse IgGs FITC.
APC/Cy7 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC/Cy7 (filled histogram), or mouse IgG2b, κ APC/Cy7 (dashed line).
PE/Cy7 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PE/Cy7 (solid line), or mouse IgG2b, κ PE/Cy7 (dashed line).
Alexa Fluor® 488 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 488 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 488 (open histogram).
Alexa Fluor® 647 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 647 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 647 (open histogram).
Pacific Blue™ anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Pacific Blue™ (filled histogram), or mouse IgG2b, κ Pacific Blue™ (open histogram).
Alexa Fluor® 700 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) Alexa Fluor® 700 (filled histogram), or mouse IgG2b, κ Alexa Fluor® 700 (open histogram).
PerCP anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PerCP (filled histogram), or mouse IgG2b, κ PerCP (open histogram).
PerCP/Cy5.5 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) PerCP/Cy5.5 (filled histogram), or mouse IgG2b, κ PerCP/Cy5.5 (open histogram).
Brilliant Violet 421™ anti-human CD20
Human peripheral blood lymphocytes were stained with CD19 PE and CD20 (clone 2H7) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 570™ anti-human CD20
Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 570™ (filled histogram), or mouse IgG2b, κ Brilliant Violet 570™ (dashed line).
Brilliant Violet 605™ anti-human CD20
Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 605™.
