The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Human CD199, also called as CCR9, is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory.
Other Names:
GPR 9-6, CDw199, Chemokine Receptor 9
Structure:
A membrane of G-protein coupled receptors and contains seven transmembrain domains. Human CCR9 has above 80% amino acid sequence homology with mouse counterpart.
Distribution:
Highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells. It is expressed to a much lower level on spleen and lymph node cells. Expression of CCR9 on gamma/delta T cells in the intraepithelial and small intestine is reported.
Ligand Receptor:
CCL25
Interaction:
CCL25
Antigen References:
1. Zaballos A et al. 1999. J Immunol 162:5671 2. Wurbel MA et al. 2007. J Immunol 178:7598 3. Wurbel MA et al. 2006. Eur J Immunol 36:73
