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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
CCR6 is a chemokine receptor that is expressed on immature dendritic cells, B lymphocytes, and memory T cells (1). CCR6 binds CCL20, although members of the β defensin family also bind CCR6 with a lower affinity (2). CCR6 positive cells and its ligand CCL20 have been detected in numerous organs, especially the secondary lymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyers patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures (3). In humans, CCR6 can function to mediate arrest of T cells on dermal endothelial cells and is highly expressed on T cells resident in both normal and psoriatic skin (4), and CCR6 and/or CCL20 have been implicated in the pathogenesis of rheumatoid arthritis(5) and inflammatory bowel disease. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 have a role in inflammatory diseases by recruiting Th17 cells to target tissues (6).
CCR6 is expressed in immature dendritic cells, B lymphocytes, and memory T cells, Th17 cells.
Function:
CCR6 has been reported to have roles in host defense and inflammation particularly at epithelial sites. CCR6 and CCL20 are important for recruitment of B cells to secondary lymphoid organs. In addition, CCR6 mediate arrest of T cells on dermal endothelial cells.
Ligand Receptor:
CCL20 (MIP-3α)
Antigen References:
1. Zaballos A, et al. 1996. Biochem Biophys Res Commun 227:846. 2. Yang D, et al. 1999. Science 286:525. 1999. 3. MacDonald KG, et al. 2007. Am J of Path 170:1229. 4. Homey B, et al.J Immun. 164:6621. 2000. 5. Hirota K, et al. 2007. J. Exp. Med. 204:2803. 6. Singh SP, et al. 2008. J Immun. 180:214.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 488 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes stained with CD19 (HIB19) APC and TG7/CCR6 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes stained with CD19 (HIB19) PE and TG7/CCR6 Alexa Fluor® 647
PerCP/Cy5.5 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes stained with CD19 (HIB19) PE and TG7/CCR6 PerCP/Cy5.5
PE anti-human CD196 (CCR6)
Human peripheral blood lymphocytes stained with CD19 (HIB19) APC and TG7/CCR6 PE
Pacific Blue™ anti-human CD196 (CCR6)
Human peripheral blood lymphocytes stained with CD19 (HIB19) PE and TG7/CCR6 Pacific Blue™
