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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescent staining and flow cytometric analysis of CCR3 expression.
CC-chemokine receptor 3 (CCR3), also known as CC CKR3, MIP1-alpha receptor like-2, eotaxin receptor, and CD193, is a member of the G protein-coupled seven transmembrane receptors family. It binds to the CC chemokines eotaxin, eotaxin-2, and eotaxin-3 with high affinity. CCR3 has also been reported to bind RANTES, MCP-3, and MCP-4 with low affinity. CCR3 receptor is expressed on human eosinophils, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor cells, Th2-like lymphocytes, and keratinocytes. CCR3 is thought to play a role in allergic diseases such as bronchial asthma and allergic rhinitis. CCR3 is a co-receptor for HIV-1 and HIV-2, and the binding of eotaxin with CCR3 has been shown to inhibit HIV infection in some cell types.
Other Names:
CC CKR3, MIP1-alpha receptor like-2, eotaxin receptor, CD193
Upregulated by high affinity Fc IgE receptor ligation (mast cells), RANTES (keratinocytes), IFNg (monocytes, neutrophils), HIV tat protein (basophils), IL-3, IL-5 and GM-CSF (CD34+ progenitor cells), IL-2 and IL-4(T cells). Downregulated by IL-3 and IL-1.
Receptors:
Eotaxin, eotaxin-2, eotaxin-3
Antigen References:
1. Gerard W, et al. 1996. J. Exp. Med. 183:2437. 2. Uguccioni C, et al. 1997. J. Clin. Invest. 100:1137. 3. Sallusto F, et al. 1997. Science 277:2005. 4. Loetscher P, et al.2001.J. Biol. Chem. 276:2986.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD193 (CCR3)
Human peripheral blood granulocytes stained with 5E8 PE and CD16 FITC
PE anti-human CD193 (CCR3)
Human peripheral blood granulocytes stained with 5E8 PE and CD16 FITC
Alexa Fluor® 647 anti-human CD193 (CCR3)
Human peripheral blood granulocytes stained with CD16 FITC and 5E8/CCR3 Alexa Fluor® 647
APC/Cy7 anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 FITC and CCR3 (clone 5E8) APC/Cy7 (top) or mouse IgG2b APC/Cy7 isotype control (bottom). Data shown was generated from granulocyte population gating.
Brilliant Violet 421™ anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 FITC and CD193/CCR3 (clone 5E8) Brilliant Violet 421™ (top) or mouse IgG2b Brilliant Violet 421™ isotype control (bottom). Data shown was generated from granulocyte population gating.
