Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
CXCR6 is a chemokine receptor that binds CXCL16. It is expressed in Th1 inflammatory diseases such as rheumatoid arthritis (3) and Grave’s disease (4) among others. Th1 cells isolated from the bronchoalveolar lavage of patients with sarcoidosis and T-cell alveolitis coexpressed CXCR3 and CXCR6. The CXCR6 ligand CXCL16 was abundantly expressed by macrophages infiltrating sarcoid tissue and/or forming the granuloma core (5). Recent data showed that CXCR6 is expressed on polymorphonuclear neutrophils in pancreatic carcinoma and in acute localized bacterial infections. CXCR6 is not constitutively expressed on PMN, but is up-regulated under inflammatory conditions and mediates migration of CXCR6-positive PMN (6). Fetus-derived trophoblasts can attract T cells, gamma delta T cells, and monocytes by producing CXCL16 and interaction with CXCR6 on these cells, leading to forming a specialized immune milieu at the maternofetal interface (7).
Other Names:
CXCR6, BONZO, CD186, STRL33, TYMSTR
Structure:
GPCR, seven transmembrane receptor
Distribution:
Th1, T-cytotoxic 1 cells, γδT cells, NK cells, and monocytes.
Function:
The receptor CXCR6 is abundantly expressed by Th1-polarized effector/memory lymphocytes accumulating at inflammatory sites. Priming of naive T cells by dendritic cells induces expression of CXCR6 on T cells. IL-12 enhances this dendritic cell-dependent upregulation, while IL-4 inhibits it (1). In addition, stimulation with IL-15 up-regulated the expression of CXCR6 on peripheral blood T cells (2).
Ligand Receptor:
CXCL13
Antigen References:
1) Kim CH, et al. 2001. J Clin Invest 107:595. 2) Nanki T, et al. 2005. Arthritis Rheum 52:3004. 3) Van der Vort R et al. 2005. Arthritis Rheum 52:1381. 4) Aust G et al. 2005. Eur J Endocrin 152:635-643. 5) Agostini C, et al. 2005. Am j Respir Crit Care Med 172:1290. 6) Gaida MM, et al. 2008. Clin Exp Immunol. 7) Huang J, et al. 2008. J. Immunol. 180:2367.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 647 anti-human CD186 (CXCR6)
Human peripheral blood mononuclear cells stimulated with IL-2 for 9 days and then stained with CD3 (UCHT1) PE and TG3/CXCR6 Alexa Fluor® 647
