Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin.8 Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301214).
CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD11a
Human peripheral blood lymphocytes stained with HI111 APC
Biotin anti-human CD11a
Human peripheral blood lymphocytes stained with biotinylated HI111, followed by Sav-PE
FITC anti-human CD11a
Human peripheral blood lymphocytes stained with HI111 FITC
LEAF™ Purified anti-human CD11a
Human peripheral blood lymphocytes stained with LEAF™ purified HI111, followed by anti-mouse IgGs FITC
PE anti-human CD11a
Human peripheral blood lymphocytes stained with HI111 PE
PE/Cy5 anti-human CD11a
Human peripheral blood lymphocytes stained with HI111 PE/Cy5
Purified anti-human CD11a
Human peripheral blood lymphocytes stained with purified HI111, followed by anti-mouse IgGs FITC
Alexa Fluor® 488 anti-human CD11a
Human peripheral blood lymphocytes stained with HI111 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD11a
Human peripheral blood lymphocytes stained with HI111 Alexa Fluor® 647
PE/Cy7 anti-human CD11a
Human peripheral blood lymphocytes were stained CD11a (clone HI111) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).
Alexa Fluor® 594 anti-human CD11a
Human neutrophils were fixed with 1% paraformaldehyde (PFA), then stained with 5 µg/ml anti-human CD11a (clone HI111) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Human peripheral blood lymphocytes were stained with CD11a (clone HI111) Alexa Flour® 594 (filled histogram) or mouse IgG1, κ Alexa Flour™ 594 isotype control (open histogram). The data was acquired by BD LSRFortessa™ cell analyzer equipped with the Yellow-Green Laser (561 nm).
Purified anti-human CD11a (MaxPar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 142Nd-anti-CD11a (HI111). Data provided by DVS Sciences.