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Alexa Fluor® 647 anti-human Basophils (2D7) Antibody
Alexa Fluor® 647 anti-human Basophils (2D7) Antibody
346203 25 tests $125.00     
346204 100 tests $295.00     
Product Details
Clone: Basophil/2D7
Isotype: Mouse IgG1, κ
Isotype Control:Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC)
Reactivity: Human
Immunogen: Lysates of purified basophils
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.



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COA:
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Application
Notes:

Additional reported applications for the relevant formats include: immunofluorescence staining, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
Application
References:

1. Kepley CL, et al. 1995. J. Immunol. 154:6548.
2. Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354.
3. Agis H, et al. 2006. J. Clin. Pathol. 59:396.
4. Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626.
5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
Human peripheral blood mononuclear cells
Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor®647


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Description:

The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.
Distribution: Basophils.
GeneID: NA
Latest Publications: View the latest Basophil articles on HighwirePress.com
UniProt: View information about Basophil on UniProt.org
Keywords: Alexa Fluor® 647 anti-human Basophils (2D7), Basophil/2D7, Alexa Fluor® 647, Human, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-human Basophils (2D7)
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor®647

  • Alexa Fluor® 647 anti-human Basophils (2D7)
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor®647

  • PE anti-human Basophils (2D7)
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were stained with CCR3 APC, fixed, permeabilized and then stained with Basophil/2D7 PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the lymphoid cell population.





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