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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
C57BL/6 splenocytes intracellularly stained with BCL/10C4 Alexa Fluor® 647 (cells were treated with BioLegend’s Foxp3 Fix/Perm buffer, cat# 421401 and then permeabilized with BioLegend’s Permeabilization Wash Buffer, cat# 421002)
Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, and endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.
Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Regulation:
Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Modification:
Phosphorylation, Ubiquitination
Antigen References:
1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214. 2. Yang E, et al. 1995. Cell 80:285. 3. Huang Z, et al. 2000. Oncogene 19:6627. 4. Deng X, et al. 2003. Blood. 102:3179.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Bcl-2
Western blot analysis of extracts from mouse splenocytes using anti-Bcl-2, clone BCL/10C4.
FITC anti-Bcl-2
C57BL/6 splenocytes intracellular stained with BCL/10C4 FITC (cells were treated with BioLegend’s Foxp3 Fix/Perm buffer, cat# 421401 and then permeabilized with BioLegend’s Permeabilization Wash Buffer, cat# 421002)
Alexa Fluor® 488 anti-Bcl-2
C57BL/6 splenocytes intracellular stained with BCL/10C4 Alexa Fluor® 488 (cells were treated with BioLegend’s Foxp3 Fix/Perm buffer, cat# 421401 and then permeabilized with BioLegend’s Permeabilization Wash Buffer, cat# 421002)
PE anti-Bcl-2
C57BL/6 splenocytes intracellularly stained with BCL/10C4 PE (cells were treated with BioLegend’s Foxp3 Fix/Perm buffer, cat# 421401 and then permeabilized with BioLegend’s Permeabilization Wash Buffer, cat# 421002)
Alexa Fluor® 647 anti-Bcl-2
C57BL/6 splenocytes intracellularly stained with BCL/10C4 Alexa Fluor® 647 (cells were treated with BioLegend’s Foxp3 Fix/Perm buffer, cat# 421401 and then permeabilized with BioLegend’s Permeabilization Wash Buffer, cat# 421002)
