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Alexa Fluor® 647 Annexin V
Alexa Fluor® 647 Annexin V
640911 25 tests $85.00     
640912 100 tests $200.00     
Product Details
Clone:
Reactivity: All mammalian species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The purified protein was conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling: The Annexin V solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this product is quality control tested by staining of apoptotic cells and analyzing by flow cytometry. The suggested use of this reagent is 5 µl per 1X105 cells in a 100 µl volume of Annexin V Binding Buffer (cat. no. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.



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Application
Notes:

Annexin V Staining
1. Wash cells twice with cold BioLegend cell staining buffer (cat # 420201) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10e6 cells/ml.
2. Transfer 100 µl of cell suspension in 5 ml test tube.
3. Add 5 µl of Alexa Fluor® 647 Annexin V.
4. Add 10 µl of PI solution (cat # 421301) or 7-AAD (cat # 420401).
5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer (cat # 422201) to each tube. Analyze by flow cytometry.
Application
References:

1. Koopman G, et al. 1994. Blood 84:1415.
2. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
3. Dachary-Prigent J et al. 1993. Blood 81:2554.
4. Lev S, et al. 2010. J. Biol Chem. 287:2771. PubMed
5. Jin D, et al. 2010. Cancer Res. 70:2245. PubMed
6. Santidrian AF, et al. 2010. Blood 116:3023. PubMed
7. Zammarchi, F., et al. 2011. PNAS. 108:17779. PubMed.
8. Liu F, et al. 2011. Cancer Res. 71:6807. PubMed.
9. Wortmann A, et al. 2011. J Biol Chem. 286:42303. PubMed.




Description:

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells.Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution (cat. no. 420401/420402) or Propidium Iodide Solution (cat. no. 421301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.
Other Names: Annexin A5
GeneID: 308
Latest Publications: View the latest Phosphatidylserine articles on HighwirePress.com
UniProt: View information about Phosphatidylserine on UniProt.org
Keywords: Alexa Fluor® 647 Annexin V, , Alexa Fluor® 647, Annexin A5 , All mammalian species, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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