This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation:
The antibody was conjugated with Alexa Fluor® 488 under optimal conditions, and is at >85% purity. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
IF
Recommended Usage:
For immunofluorescent staining applications, a concentration range of 1-4 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Chou, H-Y. E., et al., 2006. J. Biol. Chem. 281:15201.
Hela cells were stained with Alexa Fluor® 488 anti-Tubulin-alpha (10D8) mouse mAb and examined on a fluorescent scope.
Description:
Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.
Other Names:
α-tubulin, Tubulin, alpha-ubiquitous chain
Structure:
Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
Distribution:
Ubiquitous
Function:
Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
Modification:
Phosphorylation, acetylation
Interaction:
RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
Antigen References:
1. Cowan, N.J., et al., 1983. Mol. Cell. Biol. 3:1738. 2. Miller, F.D., et al., 1987. J. Cell. Biol. 105:3065. 3. Wilde, C. D., et al., 1982. Proc. Natl. Acad. Sci. USA 79:96.
