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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, immunohistochemical staining1,2 of acetone-fixed frozen sections and Zinc-fixed paraffin-embeded sections, in vitro blocking of allogeneic responses1, Macrophage stimulation3,4 and in vivo depletion.
Application References:
1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP IHC WB) 2. Kuhnlein P, et al. 1994. J. Immunol. 153:979. (IHC) 3. Hirji N, et al. 1997. J. Immunol. 158:1833. 4. Hirji N, et al. 1998. J. Immunol. 160:6004.
LOU rat splenocytes stained with 341 Alexa Fluor® 488
CD8b antigen is also known as T8, Lyt2, Ly-2, and CD8β. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Other Names:
CD8, Lyt3
Structure:
Ig superfamily, CD8β chain
Distribution:
Most thymocytes, subset of mature T cells (i.e. including most T suppressor/cytotoxic cells), macrophages
Function:
Co-receptor for TCR
Ligand Receptor:
MHC class I molecule
Antigen References:
1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. 2. Kuhnlein P, et al. 1994. J. Immunol. 153:979. 3. Hirji N, et al. 1997. J. Immunol. 158:1833. 4. Hirji N, et al. 1998. J. Immunol. 160:6004.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-rat CD8b
LOU rat splenocytes stained with 341 FITC
Purified anti-rat CD8b
LOU rat splenocytes stained with purified 341, followed by anti-mouse IgG FITC
Alexa Fluor® 488 anti-rat CD8b
LOU rat splenocytes stained with 341 Alexa Fluor® 488
Alexa Fluor® 647 anti-rat CD8b
LOU rat splenocytes stained with 341 Alexa Fluor® 647
