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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.
Application References:
1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) 2. Shioji K, et al. 2001. Circulation Res. 89:540. (IHC) 3. Mannie MD, et al. 1993. J. Immunol. 151:7293. 4. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
LOU rat splenocytes stained with W3/25 Alexa Fluor® 488
CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.
Other Names:
T4, L3T4, W3/25
Structure:
Ig superfamily, 55 kD
Distribution:
Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
Function:
T cell co-receptor, T cell activation
Ligand Receptor:
MHC class II molecule
Antigen References:
1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-rat CD4
Lou rat splenocytes stained with purified W3/25, followed by anti-mouse IgG FITC
FITC anti-rat CD4
LOU rat splenocytes stained with W3/25 FITC
PE anti-rat CD4
LOU rat splenocytes stained with W3/25 PE
APC anti-rat CD4
LOU rat splenocytes stained with W3/25 APC
Alexa Fluor® 488 anti-rat CD4
LOU rat splenocytes stained with W3/25 Alexa Fluor® 488
Alexa Fluor® 647 anti-rat CD4
LOU rat splenocytes stained with W3/25 Alexa Fluor® 647
