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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.
Distribution:
Phospho-Specific
Function:
Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-Phosphotyrosine
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phosphotyrosine antibody (clone PY-20). Lane 1, serum-starved Hela cells; Lane 2, serum-starved Hela cells following serum addition for 4 hrs. Lane 2 shows an upregulation of tyrosine phosphorylated proteins after serum addition. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Purified anti-Phosphotyrosine
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-phosphotyrosine antibody (clone PY-20). Lane 1, serum-starved Hela cells; Lane 2, serum-starved Hela cells following serum addition for 4 hrs. Lane 2 shows an upregulation of tyrosine phosphorylated proteins after serum addition. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
