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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (in the relevant formats) include: immunoprecipitation, Western blotting, immunocytochemistry, and immunohistochemical staining of acetone-fixed frozen sections2-5. Clone MECA-32 is not recommended for formalin-fixed paraffin sections.
Application References:
1. Leppink DM, et al. 1989. Transplantation 48:874. 2. Bergese SD, et al. 1994. Transplantation 57:711. (IHC) 3. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581. (IHC) 4. Kopp HG, et al. 2005. Blood 106:505. (IHC) 5. Hallmann R, et al. 1995. Dev. Dyn. 202:325. (IHC)
bEND.3 cells stained with MECA-32 Alexa Fluor® 488
MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic and the adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But in some conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and the neurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to day 16 of gestation, and disappears at day 17 and after.
Other Names:
Pan-endothelial Cell Antigen, MECA-32
Structure:
50-55 kD homodimer
Distribution:
Endothelial cell in the embryonic and the adult mice, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution
Antigen References:
1. Leppnik DM, et al. 1989. Transplantation 48:874. 2. Hallmann R, et al. 1995. Dev. Dyn. 202:325. 3. Kruse A, et al. 1999. Bio. Reprod. 61:1393.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Panendothelial Cell Antigen
bEND.3 cells stained with MECA-32 Alexa Fluor® 488
Biotin anti-mouse Panendothelial Cell Antigen
bEND.3 cells stained with MECA-32 biotin, followed by Sav-PE
