The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (in the relevant formats) include: immunoprecipitation, Western blotting, immunocytochemistry, and immunohistochemical staining2-4 of acetone-fixed frozen sections and paraffin-embedded tissue sections.
Application References:
1. Leppink, D. M., et al., 1989. Transplantation 48:874. 2. Bergese, S. D., et al. 1994. Transplantation 57:711. 3. Tanneau, G. M., et al. 1999. J. Histochem. Cytochem. 47:1581. 4. Kopp, H-G., et al. 2005. Blood 106:505.
bEND.3 cells stained with MECA-32 Alexa Fluor® 488
Description:
MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic and the adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But in some conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and the neurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to day 16 of gestation, and disappears at day 17 and after.
Other Names:
Pan-endothelial Cell Antigen, MECA-32
Structure:
50-55 kD homodimer
Distribution:
Endothelial cell in the embryonic and the adult mice, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution
Antigen References:
1. Leppnik, D.M., et al., 1989. Transplantation 48: 874. 2. Hallmann, R., et al. 1995. Dev. Dyn. 202: 325. 3. Kruse, A., et al., 1999. Bio. Reprod. 61: 1393.
4. Bergese, S. D., et al., 1994. Transplantation 57: 711.
