The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing1-4,7, immunohistochemical staining1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting1.
Application References:
1. Streeter, P. R., et al., 1988. Nature 331:41. 2. Briskin, M. J., et al., 1993. Nature 363:461. 3. Berlin, C., et al., 1993. Cell 74:185. 4. Bargatze, R. F., et al., 1995. Immunity 3:99. 5. Tanneau, G. M., et al. 1999. J. Histochem. Cytochem. 47:1581. 6. Savinov, A. Y., et al., 2003. J. Exp. Med. 197:643. 7. Rivera-Nieves, J., et al., 2005. J. Immunol. 174:2343.
Description:
MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin α4β7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. The MECA-367 antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of the mAb is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.
Other Names:
Mucosal addressin cell adhesion molecule-1
Structure:
Type I glycoprotein, Ig superfamily
Distribution:
HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas
Function:
Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand Receptor:
Integrin a4ß7, CD62L
Antigen References:
1. Streeter, P. R., et al., 1988. Nature 331: 41 2. Briskin, M.J., et al., 1993.Nature 363: 461. 3. Berlin, C., et al. 1993. Cell 74: 185. 4. Lehnert, K., et al., 1998. Eur. J. Immunol. 28: 3605. 5. Picarella, D., et al., 1997. J. Immunol. 158: 2099.
