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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3, 6 and immunohistochemistry of frozen sections4.
Most hemopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.
Other Names:
Lymphocyte antigen 6 complex, locus C
Structure:
14-16 kD protein (134 amino acid), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution:
Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
Antigen References:
1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. 2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Pacific Blue™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells stained with HK1.4 Pacific Blue™
APC anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 APC
Purified anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells stained with purified HK1.4, followed by anti-rat IgG PE (gated on myeloid cell population)
Biotin anti-mouse Ly-6C
BALB/c bone marrow cells stained with HK1.4 biotin, followed by Sav-PE
FITC anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
Alexa Fluor® 647 anti-mouse Ly-6C
BALB/c bone marrow cells stained with HK1.4 Alexa Fluor® 647 (gated on myeloid cells)
PE anti-mouse Ly-6C
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) PE (filled histogram), or rat IgG2c, κ PE isotype control (open histogram). Gated on myeloid cell population.
PerCP/Cy5.5 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 PerCP/Cy5.5
PE/Cy7 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 PE/Cy7
Alexa Fluor® 488 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with anti-mouse Ly-6C, HK1.4 Alexa Fluor® 488
Alexa Fluor® 700 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 Alexa Fluor® 700
APC/Cy7 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 APC/Cy7
PerCP anti-mouse Ly-6C
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) PerCP. Data shown was gated on myeloid cell population.
Brilliant Violet 570™ anti-mouse Ly-6C
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 570™ (filled histogram) or rat IgG2a, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.
