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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
ELISA or ELISPOT Detection1,9,11: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. No. 504902/504904) as the capture antibody.
Neutralization: The JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Application References:
1. Simkin G, et al. 2000. J. Immunol. 164:2457. 2. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260. 3. Khanna A, et al. 2000. J. Immunol. 164:1346. 4. Sander B, et al. 1993. J. Immunol. Methods 166:201. 5. Litton M, et al. 1994. J. Immunol. Methods 175:47. 6. Andersson U, et al. 1999. Detection and qunatification of gene expression. New York:Springer-Verlag. 7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28. 8. Wang W, et al. 2004. FASEB J. 18:1043. 9. Brummel R and Lenert P. 2005. J. Immunol. 174:2429. 10. Lawson BR, et al. 2007. J. Immunol. 178:5366. 11. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed 12. Brummel R, et al. 2005. J. Immunol.174:2429. PubMed 13. Kang YJ, et al. 2007. Stem Cells 25:1814. PubMed
Description:
IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.
Other Names:
Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF)
Structure:
Acid-labile cytokine, dimer, 17-21 kD (Mammalian)
Regulation:
Downregulated by IL-4, IL-10
Cellular Sources:
Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cellular Targets:
T cells, B cells, mast cells, macrophages
Receptors:
IL-10R (CDw210)
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314. 3. Howard M, et al. 1992. Immunol. Today 13:198. 4. Quesniaux V. 1992. Res. Immunol. 143:385. 5. Norton SK, et al. 2008. J. Immunol. 180:2848.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
