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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The KH74 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The KH74 antibody cross-reacts with the I-Af, I-Ar, I-As, and I-Av haplotypes.
Other Names:
MHC class II
Structure:
MHC class II
Distribution:
B and activated T cells, APCs of H-2b mice
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD4
Antigen References:
1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Alexa Fluor® 488 anti-mouse I-Ab, KH74, Alexa Fluor® 488, AF488, MHC class II, Mouse b haplotype, Cross-reacts with the I-A f,r,s,v haplotypes. Does not react with other haplotypes (e.g., a, d, k, n, p, q, u), Flow Cytometry, Immunofluorescence microscopy, Immunohistochemistry, Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse I-Ab
C57BL/6 splenocytes stained with KH74 FITC
FITC anti-mouse I-Ab
C57BL/6 splenocytes stained with KH74 FITC
Purified anti-mouse I-Ab
C57BL/6 splenocytes stained with KH74 FITC
Alexa Fluor® 488 anti-mouse I-Ab
C57BL/6 splenocytes stained with KH74 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with KH74 Alexa Fluor® 647
