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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections,and inhibition of TLR2-dependent cell activation in vitro and in vivo1.
Application References:
1. Meng G, et al. 2004. J. Clin. Invest. 113:1473.
Human peripheral blood monocytes stained with T2.5 Alexa Fluor® 488
Toll-like receptors (TLRs) are type I transmembrane signaling receptors characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain. TLRs are responsible for recognizing pathogen-associated molecule patterns (PAMPs). They are primary sensors of both innate and adaptive immune systems. To date a total of 12 TLRs have been indentified. Toll-like receptor 2 (TLR2), known as CD282, is one of the important receptors. It is expressed on monocytes/macrophages, dendritic cells, granulocytes, endothelial and epithelial cells. By association with TLR1 or TLR6 and CD36, TLR2 is central to the innate immune response to diverse set of pathogens, including several components of Gram-positive bacteria such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi. The T2.5 antibody reacts with both mouse and human TLR2. It is able to inhibit cell activation through blocking the interaction of TLR2 with its ligands.
Other Names:
CD282, TLR-2, TLR2
Structure:
Type I transmembrane protein characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain, associated with TLR1 or TLR6 and CD36
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse/human CD282 (TLR2)
Human peripheral blood monocytes stained with biotinylated T2.5, followed by Sav-PE
FITC anti-mouse/human CD282 (TLR2)
Human peripheral blood monocytes stained with T2.5 FITC
Alexa Fluor® 488 anti-mouse/human CD282 (TLR2)
Human peripheral blood monocytes stained with T2.5 Alexa Fluor® 488
