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The antibody was conjugated with Alexa Fluor® 488 under optimal conditions, and is at >85% purity. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats)include: immunoprecipitation1, Western blotting1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
Application References:
1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565.
The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15.The expression pattern of SSEA-1 antigen is different from human and mouse. In mouse, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In human, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.
Other Names:
Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1
Structure:
Carbohydrate epitope, Lewis blood group antigens
Distribution:
Murine embryonic stem cells (ES), embryonal carcinoma cells, 8-cell to blastocyst embryos; human differentiated cells, not on undifferentiated ES cells
Function:
Adhesion, differentiation
Antigen References:
1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565. 2. Harris JF, et al. 1984. J. Immunol. 132:2502. 3. Gooi HC, et al. 1981. Nature 292:156. 4. Cui L, et al. 2004. J. Histochem. Cytochem. 52:1447.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse/human CD15 (SSEA-1)
F9 (mouse embryonic carcinoma cell line) stained with MC-480 PE
Biotin anti-mouse/human CD15 (SSEA-1)
F9 (mouse embryonic carcinoma cell line) stained with biotinylated MC-480, followed by Sav-PE
Purified anti-mouse/human CD15 (SSEA-1)
F9 (mouse embryonic carcinoma cell line) stained with purified MC-480, followed by anti-mouse IgM-PE
Human peripheral blood lymphocytes, monocytes, granulocytes stained with purified MC-480, followed by anti-mouse IgM PE
