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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 120216).
Application References:
1. Cobbold SP, et al. 2004. J. Immunol. 172:6003. 2. Biburger M, et al. 2008. J. Leukocyte Biol. 84:264. PubMed
BALB/c mouse splenocytes stained with PE anti-muse CD25 (PC61) and Alexa Fluor® 488 rat IgG2b, k isotype control (bottom) or Alexa Fluor® 488 YGITR 765 (upper)
GITR, Glucocorticoid-induced TNFR-related gene, is a member of the TNF receptor superfamily, also known as TNFRSF18, and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD4+CD25+ T regulatory (Treg) cells. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation, and abrogate the inhibitory functions of CD4+CD25+ Treg cells. In vivo activation GITR causes development of autoimmune diseases and restores the suppressed immune responses.
Other Names:
TNFRSF18, AITR
Structure:
TNF receptor superfamily member
Distribution:
Low levels on resting T cells, high on CD4+CD25+ Treg cells, expression upregulated upon activation.
Function:
Co-stimulate GITR expressing cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.
Ligand Receptor:
GITR ligand (TNFSF18)
Antigen References:
1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059 2. Ronchetti S, et al. 2004. Eur. J. Immunol. 34:613 3. Kanamaru F, et al. 2004. J. Immunol. 172:613 4. Shimizu J, et al. 2002. Nat. Immunol. 3:135 5. Stephens GL, et al. 2004. J. Immunol. 173:5008 6. McHugh RS, et al. 2002. Immunity 16:311
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD357 (GITR)
C57BL/6 mouse splenocytes stained YGITR 765 FITC and CD25 APC
PE anti-mouse CD357 (GITR)
C57BL/6 mouse splenocytes stained with CD4 APC and YGITR 765 PE
Alexa Fluor® 488 anti-mouse CD357 (GITR)
BALB/c mouse splenocytes stained with PE anti-muse CD25 (PC61) and Alexa Fluor® 488 rat IgG2b, k isotype control (bottom) or Alexa Fluor® 488 YGITR 765 (upper)
Alexa Fluor® 647 anti-mouse CD357 (GITR)
C57BL/6 Mouse splenocytes stained with YGITR765 Alexa Fluor® 647
PE/Cy5 anti-mouse CD357 (GITR)
C57BL/6 Mouse splenocytes stained with YGITR765 PE/Cy5
PE/Cy7 anti-mouse CD357 (GITR)
C57BL/6 Mouse splenocytes stained with YGITR765 PE/Cy7
