The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested IHC(Frozen)1,4*, IF* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining1,4 of acetone-fixed frozen sections, in vitro stimulation of T cells1 and depletion2 of CD3+ cells in vivo. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100208).
Application References:
1. Miescher, G.C., et al., 1989. Immunol. Lett. 23:113. 2. Mysliwietz, J., et al., 1992. Blood 80:2661. 3. Wu, L., et al., 1991. J. Exp. Med. 174:1617. 4. Zhang, Y., et al., 2002. J. Immunol. 168: 3088. 5. Zan, H., et al., 2005. EMBO J. 24: 3757. 6. Xiao,S.,et al.2007.J Exp Med.204:1691.PubMed
C57BL/6 mouse splenocytes stained with 17A2 Alexa Flour® 488
Description:
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 17A2 antibody recognizes ε/γ (but not ε/δ) of CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo.
Other Names:
T cell antigen receptor complex, T3
Structure:
Ig superfamily, CD3/TCR, 20 kD
Distribution:
Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function:
Antigen recognition, TCR signal transduction, T cell activation
Ligand Receptor:
Peptide antigen/MHC-complex
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Davis, M.M. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss, A. et al., 1994. Cell 76:263.
