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Alexa Fluor® 488 anti-mouse CD206 (MMR) Antibody
Cat. # Size Price  

141709 25 µg $110.00
    
141710 100 µg $250.00
    
Product Details

Clone: C068C2 (See other available formats)
Isotype: Rat IgG2a, κ
Isotype Control:Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: Recombinant mouse CD206 (MMR)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC, ICFC - Quality tested
IHC, IF - Validated
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5-5 µg/ml is suggested. For immunofluorescence microscopy, a concentration range of 2.5-10 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation
Laser:
Blue Laser (488 nm)
Application
Notes:
Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other.
Application
References:
  Publication Library
 Image 1
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).

 Image 2


Compare all formats

See Alexa Fluor® 488 spectral data





 


Antigen Details

Description: CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.
Other Names: MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Structure: Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution: Macrophages, dendritic cells, Langerhans cells, liver endothelial cells
Function: Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand Receptor: Antigen containing mannose, fucose, or an N-acetyl glucosamine
Antigen
References:
1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.
GeneID: 17533
UniProt: View information about CD206 on UniProt.org
Keywords: Alexa Fluor® 488 anti-mouse CD206 (MMR), C068C2, Alexa Fluor® 488, AF488, MMR (macrophage mannose receptor), MR (mannose receptor), MRC1, Mouse, Flow Cytometry, Intracellular Staining for Flow Cytometry, Immunohistochemistry, Immunofluorescence microscopy, Immunology, Antibodies
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Related Protocols

Cell Surface Immunofluorescence Staining Protocol
Immunohistochemistry Protocol for Frozen Sections
Immunofluorescence Microscopy Protocol
Intracellular Flow Cytometry Staining Protocol
Additional Data

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Version: 4 Revision Date: 2014-06-27
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD206 (MMR)
    Thioglycollate-elicited Balb/c peritoneal macrophages were

    Thioglycollate-elicited Balb/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with biotinylated CD206 (clone C068C2) (top) or rat IgG2a, κ isotype control (bottom), followed by Sav-PE.





  • Purified anti-mouse CD206 (MMR)
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages

    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with purified CD206 (clone C068C2) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.

  • FITC anti-mouse CD206 (MMR)
    Thioglycollate-elicited Balb/c macrophages were fixed/pemeabilized,

    Thioglycollate-elicited Balb/c macrophages were fixed/pemeabilized, and then stained with CD107b (Mac-3) APC and CD206 (clone C068C2) FITC (top) or rat IgG2a, κ FITC isotype control (bottom).





  • PE anti-mouse CD206 (MMR)
    Thioglycollate-elicited BALB/c peritoneal macrophages were

    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) PE (top) or rat IgG2a, κ PE isotype control (bottom).





  • APC anti-mouse CD206 (MMR)
    Thioglycollate-elicited BALB/c peritoneal macrophages were

    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).





  • Alexa Fluor® 488 anti-mouse CD206 (MMR)
    Thioglycollate-elicited BALB/c peritoneal macrophages were

    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) APC, and then intracellularly stained with CD206 (clone C068C2) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).





  • Alexa Fluor® 647 anti-mouse CD206 (MMR)
    Thioglycollate-elicited BALB/c peritoneal macrophages were

    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).





  • PerCP/Cy5.5 anti-mouse CD206 (MMR)
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages

    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with CD206 (clone C068C2) PerCP/Cy5.5 (filled histogram) or rat IgG2a, κ PerCP/Cy5.5 isotype control (open histogram).

  • PE/Cy7 anti-mouse CD206 (MMR)
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages

    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) PE/Cy7 (top) or rat IgG2a, κ PE/Cy7 isotype control (bottom).





  • Brilliant Violet 421™ anti-mouse CD206 (MMR)
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages

    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were intracellularly stained with CD206 (clone C068C2) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).

    C57BL/6 mouse frozen lymph node

    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the cells were stained with 5 µg/ml of CD206 (clone C068C2) Brilliant Violet 421™ (blue), 5 µg/ml of CD3 (clone 145-2C11) Alexa Fluor® 647 (green), and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594 (red) overnight at 4°C. The image was captured by 10X objective.

  • Brilliant Violet 605™ anti-mouse CD206 (MMR)
    Thioglycollate-elicited Balb/c peritoneal macrophages were

    Thioglycollate-elicited Balb/c peritoneal macrophages were fixed, permeabilized, then intracellularly stained with CD107b (Mac-3) FITC and CD206 (clone C068C2) Brilliant Violet 605™ (top) or rat IgG2a, κ Brilliant Violet 605™ isotype control (bottom).





  • Brilliant Violet 650™ anti-mouse CD206 (MMR)
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages

    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) Brilliant Violet 650™ (top) or rat IgG2a, κ Brilliant Violet 650™ isotype control (bottom).





  • Alexa Fluor® 594 anti-mouse CD206 (MMR)
    C57BL/6 mouse frozen spleen section

    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD206 (clone C068C2) Alexa Fluor® 594 (red), 2.5 µg/ml of CD3 (clone 145-2C11) Alexa Fluor® 647 (green), and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. The image was captured by 10X objective.

  • Brilliant Violet 711™ anti-mouse CD206 (MMR)
    Thioglycollate-elicited Balb/c peritoneal macrophages were

    Thioglycollate-elicited Balb/c peritoneal macrophages were fixed, permeabilized, then intracellularly stained with CD107b (Mac-3) PE and CD206 (Clone C068C2) Brilliant Violet 711™ (top) or rat IgG2a, κ Brilliant Violet 711™ isotype control (bottom).





  • Brilliant Violet 785™ anti-mouse CD206 (MMR)
    Thioglycollate-elicited Balb/c peritoneal macrophages were

    Thioglycollate-elicited Balb/c peritoneal macrophages were fixed, permeabilized, then intracellularly stained with CD107b (Mac-3) PE and CD206 (clone C068C2) Brilliant Violet 785™ (top) or rat IgG2a, κ Brilliant Violet 785™ isotype control (bottom).





  • PE/Dazzle™ 594 anti-mouse CD206 (MMR)
    Thioglycollate-elicited Balb/c peritoneal macrophages were

    Thioglycollate-elicited Balb/c peritoneal macrophages were fixed, permeabilized, then intracellularly stained with CD107b Alexa Fluor® 647 and CD206 (clone C068C2) PE/Dazzle™ 594 (top image) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (bottom image).





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