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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The TC15-12F12.2 antibody has been reported to enhance the production of IFN-γ by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion1, enhancing IFN-γ production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 115906).
Application References:
1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC Costim IP) 2. Forsberg EC, et al. 2005. PLoS Genet. 1:281. (FC) 3. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (FC) 4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC) 5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC) 6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC Block) PubMed 7. Jung Y, et al. 2007. Blood 110:82. PubMed 8. Pimanda JE, et al. 2007. P. Natl. Acad. Sci. USA 104:840. 9. Sugiyama T, et al. 2007. P. Natl. Acad. Sci. USA 104:175. 10. Injune K, et al.2006. Blood 108:737. PubMed 11. Ema H, et al.2006. Nat Protoc.1:2979. PubMed 12. Fraser ST, et al.2007. Blood 109:4616. PubMed 13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed 14. Song J, et al. 2010. Blood 115:2592. PubMed 15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed 16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) Alexa Fluor® 488 (filled histogram) or rat IgG2a Alexa Fluor® 488 isotype control (open histogram).
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) Alexa Fluor® 488 (filled histogram) or rat IgG2a Alexa Fluor® 488 isotype control (open histogram) (gated on lymphoid cell population).
CD150 is a 75-95 kD member of the immunoglobulin superfamily and is also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with purified CD150 (clone TC15-12F12.2) (filled histogram) or rat IgG2a isotype control (open histogram), followed by anti-rat IgG FITC.
PE anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE (filled histogram) or rat IgG2a PE isotype control (open histogram) (gated on lymphoid cell population).
LEAF™ Purified anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with LEAF™ purified CD150 (clone TC15-12F12.2) (filled histogram) or rat IgG2a isotype control (open histogram), followed by anti-rat IgG FITC.
Biotin anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with biotinylated CD150 (clone TC15-12F12.2) (filled histogram) or rat IgG2a isotype control (open histogram), followed by Sav-PE.
APC anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) APC (solid line) or rat IgG2a APC isotype control (broken line).
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) APC (filled histogram) or rat IgG2a APC isotype control (open histogram) (gated on lymphoid cell population).
PE/Cy5 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE/Cy5 (filled histogram) or rat IgG2a PE/Cy5 isotype control (open histogram).
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE/Cy5 (filled histogram) or rat IgG2a PE/Cy5 isotype control (open histogram) (gated on lymphoid cell population).
PE/Cy7 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE/Cy7 (filled histogram) or rat IgG2a PE/Cy7 isotype control (open histogram).
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE/Cy7 (filled histogram) or rat IgG2a PE/Cy7 isotype control (open histogram) (gated on lymphoid cell population).
Alexa Fluor® 488 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) Alexa Fluor® 488 (filled histogram) or rat IgG2a Alexa Fluor® 488 isotype control (open histogram).
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) Alexa Fluor® 488 (filled histogram) or rat IgG2a Alexa Fluor® 488 isotype control (open histogram) (gated on lymphoid cell population).
Alexa Fluor® 647 anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) Alexa Fluor® 647 (filled histogram) or rat IgG2a Alexa Fluor® 647 isotype control (open histogram).
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) Alexa Fluor® 647 (filled histogram) or rat IgG2a Alexa Fluor® 647 isotype control (open histogram) (gated on lymphoid cell population).
PerCP/Cy5.5 anti-mouse CD150 (SLAM)
C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PerCP/Cy5.5 (filled histogram) or rat IgG2a PerCP/Cy5.5 isotype control (open histogram) (gated on lymphoid cell population).
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PerCP/Cy5.5 (filled histogram) or rat IgG2a PerCP/Cy5.5 isotype control (open histogram).
Pacific Blue™ anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) Pacific Blue™ (filled histogram) or rat IgG2a Pacific Blue™ isotype control (open histogram).
Brilliant Violet 421™ anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with SLAM (clone TC15-12F12.2) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
C57BL/6 mouse bone marrow cells were stained with SLAM (clone TC15-12F12.2) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on the lymphoid cell population.
