The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC4 - Quality tested IHC(Frozen)3*, IF* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application Notes:
Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of cell-cell adhesion1,2, and immunohistochemical staining3 of acetone-fixed frozen sections. The M17/4 antibody does not block the binding of 2D7 antibody (Cat. No. 101002) to CD11a. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 101110).
Application References:
1. Sanchez-Madrid, F., et al., 1982. Cell Immunol. 73:1. 2. Kuhlman, P., et al., 1991. J. Immunol. 146:1773. 3. Mizgerd, J. P., et al., 1997. J. Exp. Med. 186:1357. 4. Hailman, E. and Allen, P. M., 2005. J. Immunol. 175:4847.
BALB/c mouse splenocytes stained with M17/4 Alexa Fluor® 488
Description:
CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50). The M17/4 antibody can block CD11a-mediated cellular adhesion.
Other Names:
αL integrin, LFA-1 α subunit, Ly-15, Ly-21
Structure:
Integrin family, associates with integrin ß2 (CD18), 180 kD
Distribution:
Lymphocytes, granulocytes, monocytes, macrophages
Function:
Cellular adhesion
Ligand Receptor:
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Springer, T.A. 1994. Cell 76:301. 3. Lub, M., et al., 1995. Immunol. Today 16:479.
