The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC1,2 - Quality Tested IHC(Frozen)*, IF * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that reagents be titrated for optimal performance in the particular application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections, and in vitro costimulation1,3,4 of T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 313512).
Application References:
1. Redoglia, V., et al. 1996. Eur. J. Immunol. 26:2781. 2. Yagi, J., et al. 2003. J. Immunol. 171:783. 3. Arimura, Y., et al. 2002. Int. Immunol. 14:555. 4. Arimura, Y., et al. 2004. J. Biol. Chem. 279:11408.
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with C398.4A Alexa Fluor® 488
Description:
The C398.4A antibody reacts with the human, mouse and rat 47-57 kD ICOS protein, also known as inducible costimulatory molecule and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation.
Other Names:
Inducible COStimulatory molecule, H4, CD278
Structure:
CD28/CTLA-4, 47-57 kD
Distribution:
Activated T cells, subset of thymocytes
Function:
Costimulates T cell activation, proliferation, humoral immune response
Ligand Receptor:
B7h/B7RP-1/GL-50
Antigen References:
1. Redoglia, V., et al. 1996. Eur. J. Immunol. 26:2781. 2. Hutloff, A., et al. 1999. Nature 397:263. 3. Buonfiglio, D., et al. 2000. Eur. J. Immunol. 30:3463. 4. Coyle, A. J., et al. 2000. Immunity 13:95.
