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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin α61,4, and immunohistochemistry2,3,5 of acetone-fixed frozen sections.
Application References:
1. Georas SN, et al. 1993. Blood 82:2872. 2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. 3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. 4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. 5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. 6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709. 7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed 8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed 9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed 10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed 11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed 12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GoH3) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).
CD49f is a 120 kD integrin family member, also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin α6 function in vivo.
Other Names:
VLA-6 α chain, α6 integrin, integrin α6
Structure:
Integrin family, associates with ß1 or ß4, 120 kD
Distribution:
Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells
Function:
Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
Ligand Receptor:
With integrin ß1 (CD29) forms VLA-6, with integrin ß4 (CD104) forms a6ß4 integrin; laminin and kalinin are ligands for these receptors
Antigen References:
1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145. 2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207. 3. Aumailly MR, et al. 1990. Exp. Cell Res. 188:55. 4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human/mouse CD49f
Human peripheral blood lymphocytes stained with purified GoH3, followed by anti-rat IgGs FITC
Biotin anti-human/mouse CD49f
C57BL/6 mouse splenocytes stained with biotinylated GoH3, followed by Sav-PE
FITC anti-human/mouse CD49f
Human peripheral blood lymphocytes stained with GoH3 FITC
Alexa Fluor® 488 anti-human/mouse CD49f
Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GoH3) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).
Alexa Fluor® 647 anti-human/mouse CD49f
Human peripheral blood lymphocytes stained with GoH3 Alexa Fluor® 647
LEAF™ Purified anti-human/mouse CD49f
Human peripheral blood lymphocytes stained with LEAF™ purified GoH3, followed by anti-rat IgG FITC
PE anti-human/mouse CD49f
Human peripheral blood lymphocytes stained with GoH3 PE
APC anti-human/mouse CD49f
Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GOH3) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
PerCP/Cy5.5 anti-human/mouse CD49f
Human peripheral blood lymphocytes stained with GoH3 PerCP/Cy5.5
Pacific Blue™ anti-human/mouse CD49f
Human peripheral blood lymphocytes stained with GoH3 Pacific Blue™
