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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® 488 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 488 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
PMA + ionomycin-stimulated (6 hours in the presence of monensin) human peripheral blood lymphocytes surface stained with CD3 APC, fixed and permeabilized and stained with IL-17F (clone Poly5166) Alexa Fluor® 488 (top) or blocked by pre-incubation with purified IL-17F (Poly5166) (bottom).
IL-17F is part of the IL-17 cytokine family which consists of at least seven structurally related proteins (IL-17A, B, C, D, E, F, and A/F). IL-17F homodimer adopts a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for inter-chain bonding. IL-17F is most closely related to IL-17A, sharing 50% amino acid sequence homology. It is expressed by Th17 cells, along with IL-17A homodimer and IL-17A/F heterodimer. IL-17F has been shown to inhibit endothelial cell angiogenesis and induce increased production of IL-2, TGF-β, and MCP-1, playing a critical role in the regulation of inflammatory reactions.
Other Names:
Interleukin-17F, IL-17F
Structure:
IL-17F is a homodimeric, 34 kD member of the IL-17 family, which includes IL-17(A), B, C, D, E (IL-25).
Distribution:
Epithelial ,endothelial cells, keratinocytes, fibroblasts, and granulocytes.
Function:
IL-17F can induce target cells to produce proinflammatory cytokines such as IL-1β, IL-6, G-CSF, GM-CSF, and TNF and chemokines including CXCL1/Gro-α, CXCL2/Gro-β, and CXCL8/IL-8 that attract and activate leukocytes, eg, neutrophils. Th17 and other IL-17F-producing cells play protective roles in the clearance of extracellular pathogens, including bacteria and fungi. IL-17F can also play adverse roles in inflammation associated with asthma and autoimmune diseases.
Ligand Receptor:
IL-17R cell surface receptor
Interaction:
IL-17F forms a heterodimer with IL-17A
Antigen References:
1. Fouser L, et al. 2008. Immunol. Rev. 226:87. 2. Shen F, et al. 2008. Cytokine 41:92. 3. Starnes T, et al. 2001. J. Immunol. 167:4137. 4. Lee YK, et al. 2009. Immunity 30:9.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 488 anti-human IL-17F
PMA + ionomycin-stimulated (6 hours in the presence of monensin) human peripheral blood lymphocytes surface stained with CD3 APC, fixed and permeabilized and stained with IL-17F (clone Poly5166) Alexa Fluor® 488 (top) or blocked by pre-incubation with purified IL-17F (Poly5166) (bottom).
