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Alexa Fluor® 488 anti-human CD8a Antibody
Alexa Fluor® 488 anti-human CD8a Antibody
300916 100 tests $225.00 Qty:
    

Product Details

 
Clone: HIT8a (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC)
Workshop
Number:
V CD08.10
Reactivity: Human, Cross-Reactivity: Chimpanzee, Marmoset
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Application
Notes:

Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.

Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6.

Application
References:

  Publication Library
Alexa Fluor® 488 anti-human CD8a Antibody HIT8a Image 1
Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor® 488


Compare all formats

See Alexa Fluor® 488 spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Other Names: T8, Leu2
Structure: Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution: Majority of thymocytes, T cell subset, NK cells
Function: MHC class I co-receptor, thymic differentiation, T cell activation
Ligand Receptor: MHC Class I molecules
Antigen
References:

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

GeneID: 925
Latest Publications: View the latest CD8a articles on HighwirePress.com
UniProt: View information about CD8a on UniProt.org
Keywords: Alexa Fluor® 488 anti-human CD8a, HIT8a, Alexa Fluor® 488, AF488, T8, Leu2, Human, Cross-Reactivity: Chimpanzee, Marmoset , Flow Cytometry, Immunology, Antibodies


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Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC


Other Formats

DescriptionCloneApplications
Alexa Fluor® 647 anti-human CD8aHIT8aFC
Alexa Fluor® 700 anti-human CD8aHIT8aFC
APC anti-human CD8aHIT8aFC
APC/Cy7 anti-human CD8aHIT8aFC
Biotin anti-human CD8aHIT8aFC
FITC anti-human CD8aHIT8aFC
Pacific Blue™ anti-human CD8aHIT8aFC
PE anti-human CD8aHIT8aFC
PE/Cy5 anti-human CD8aHIT8aFC
PE/Cy7 anti-human CD8aHIT8aFC
PerCP anti-human CD8aHIT8aFC
PerCP/Cy5.5 anti-human CD8aHIT8aFC
Purified anti-human CD8aHIT8aFC, IHC


Related Pathways

Pathway
Dendritic Cells
Gamma-Delta T cells


Related Categories

Category
Immunology > Non-Human Primate Immunology > CD8
Immunology > Human Immunology > T Regulatory Cells (TReg) > CD8 (T8)
Immunology > Human Immunology > Th1/Th2 > CD8a (T8, Leu2) > HIT8a
Immunology > Human Immunology > Th17 > CD8a > HIT8a
Immunology > Human Immunology > Dendritic cells > CD8a > HIT8a
Immunology > Human Immunology > Costimulatory Molecules > CD8a > HIT8a
Immunology > Human Immunology > CD and Related Molecules > CD8a (T8, Leu2) > HIT8a
Immunology > Human Immunology > NK/NKT > CD8a > HIT8a
Immunology > Human Immunology > Stem Cells > CD8 (T8) > HIT8a

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 4 Revision Date: 2013-04-25
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a FITC

  • Biotin anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated HIT8a, followed by Sav-PE

  • FITC anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a FITC

  • PE anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a PE

  • PE/Cy5 anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a FITC

  • Purified anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified HIT8a, followed by anti - mouse IgGs FITC

  • PE/Cy7 anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a PE/Cy7

  • Alexa Fluor® 488 anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor® 647

  • Alexa Fluor® 700 anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor® 700

  • PerCP anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a PerCP

  • PerCP/Cy5.5 anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a PerCP/Cy5.5

  • APC/Cy7 anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a APC/Cy7

  • Pacific Blue™ anti-human CD8a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HIT8a Pacific Blue™

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