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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 305212).
CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
Other Names:
B7-1, B7, BB1
Structure:
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution:
Activated B and T cells, macrophages, dendritic cells
Function:
T cell costimulation
Ligand Receptor:
CD28, CD152 (CTLA-4)
Antigen References:
1. Freeman G, et al. 1991. J. Exp. Med. 174:625. 2. Linsley P, et al. 1996. Immunity 4:535. 3. Linsley P, et al. 1991. J. Exp. Med. 174:561.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD80
Daudi (human B Burkitt's lymphoma cell line) cells stained with 2D10 PE
FITC anti-human CD80
Human B-cell Burkitt's lymphoma cell line Daudi stained with 2D10 FITC
LEAF™ Purified anti-human CD80
Daudi (human B Burkitt's lymphoma cell line) cells stained with 2D10 PE
PE anti-human CD80
Human B-cell Burkitt's lymphoma cell line Daudi stained with 2D10 PE
PE/Cy5 anti-human CD80
Daudi (human B Burkitt's lymphoma cell line) cells stained with 2D10 PE
Purified anti-human CD80
Daudi (human B Burkitt's lymphoma cell line) cells stained with 2D10 PE
Alexa Fluor® 488 anti-human CD80
Human B-cell Burkitt's lymphoma cell line Daudi stained with 2D10 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD80
Human B-cell Burkitt's lymphoma cell line Daudi stained with 2D10 Alexa Fluor® 647
PE/Cy7 anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji stained with 2D10 PE/Cy7
APC anti-human CD80
Daudi (human B Burkitt's lymphoma cell line) cells stained with 2D10 APC
Brilliant Violet 650™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line, Raji was stained with CD80 (clone 2D10) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).
Brilliant Violet 421™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji was stained with CD80 (clone 2D10) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 605™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji was stained with CD80 (clone 2D10) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).
