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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and functional assay. The LEAF™ format is suggested for functional studies.
Application References:
1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. 2. Kirkland SC, et al. 2008. J. Biol. Chem. 283:27612. PubMed 3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood platelets stained with AK-7 Alexa Fluor® 488
CD49b is a 170 kD transmembrane protein, also known as α2 integrin, VLA-2 α chain, Integrin α2 or GPIa. It associates with CD29 (β1 integrin) to form VLA-2, a collagen and alminin receptor on many cell types including monocytes, platelets, activated T cells, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts. CD49b has been reported to interact with F-actin and matrix metalloproteinase 1. CD49b is a platelet alloantigen and has been associated with neonatal alloimmune thrombocytopenia. Deficiencies in this protein have been associated with hemorrhagic disorders.
Other Names:
α2 integrin, VLA-2 α chain, Integrin α2 chain
Structure:
Integrin alpha chain family, Type I membrane protein, alpha chain of heterodimeric integrin receptor, 170 kD. Associates with CD29 to form collagen, laminin receptor
Distribution:
Broadly expressed on many cell types including B cells, monocytes, platelets, megakaryocytes, neuronal cells, epithelial cells, osteoclasts
Function:
With CD29 binds collagen and laminin
Ligand Receptor:
With integrin ß1 (CD29) forms receptor for laminin and collagen
Antigen References:
1. Kaplan C, et al. 1991. Br. J. Haemat. 78:425 2. Kiefel V, et al. 1991. Vox Sang. 60:244. 3. Nieuwenhuis HK, et al. 1985. Nature 318:470. 4. Takada Y, et al. 1989. J. Cell Biol. 109:397.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD49b
Human peripheral blood platelets stained with purified AK-7, followed by anti-mouse IgGs FITC
FITC anti-human CD49b
Human peripheral blood platelets stained with AK-7 FITC
Alexa Fluor® 488 anti-human CD49b
Human peripheral blood platelets stained with AK-7 Alexa Fluor® 488
